ARCHIVES(Journal of Harmonized Research in Pharmacy)


Title: SOME BIOCHEMICAL PARAMETERS OF MALE RATS TREATED WITH PROTEASE EXTRACTED FROM VENOM OF ECHIS CARINATUS SNAKE
Author: Amenah S. Kadhim, Mohammed A. Awda, Khalid G. Al-Fartosi
Keyword: Echis carinatus, Protease, Biochemical parameters.
Page No: 23-29
Abstract: This work aimed to study the effect of extracted protease from Echis carinatus venom on some biochemical parameters of male rats which including total protein, albumin, MDA, total cholesterol, trigl ycerides, HDL, LDL, VLDL, alanine amino transferase(ALT) and aspartate amino transferase (AST) in male rats . Isolation and purification of protease from Echis carinatus venom were carried out by using combination of gel filtration and ion exchange chromatography. After that, three doses were used (0.05, 0.1 , 0.2) ml of purified protease and the animals were injected (I.P) for 14 days as one dose daily. The results showed that purified protease of Echis carinatus venom caused a significant decrease (P< 0.05 ) in serum total protein, albumin and HDL while MDA, the total cholesterol, triglycerides, LDL, VLDL, ALT and AST increased significantly(P<0.05). Kew words: Echis carinatus, Protease, Biochemical parameters. Download PDF


Title: DOCTOR OF PHARMACY EDUCATION IN INDIA - STRENGTHS, WEAKNESSES AND OPPORTUNITIES, A CRITICAL ANALYTICALSTUDY BASED ON FIVE YEARS EXISTENCE IN INDIA.
Author: Revikumar K.G, Veena R.
Keyword: Doctor of Pharmacy, Pharm.D, Indian Pharm.D education, Pharm.D critical study.
Page No: 30-47
Abstract: The post 1920 period, particularly the 1940 to 1970s, witnessed many scientific developments and achievements in the area of Hospital pharmacy helping the evolvement of Clinical Pharmacy in USA. Clinical Pharmacy denotes the practice of pharmacy near the bed side of the patient. It is practiced by both hospital and community pharmacists. The Doctor of Pharmacy (Pharm.D) degree, is a professional doctor degree in Pharmacy and is a global program in pharmacy education.The first effort to introduce Pharm.D in India was initiated in Trivandrum in 1999 when University of Kerala approved the syllabus and regulations, framed by K.G.Revikumar , the head of Hospital and Clinical Pharmacy, of Trivandrum Medical College. The program could not be started for certain reasons. In 2008 the Pharmacy council of India managed to introduce a six year regular Pharm D and the three year post baccalaureate Pharm.D in India. By 2013 November, the PCI had given approval to over 140 institutions covering states like Kerala, Tamil Nadu, Karnataka, Andhra Pradesh, Maharashtra, Gujarat, Rajasthan, UP and Punjab for starting Pharm.D in India. The first batch of regular Pharm.D will graduate by the end of 2014. The strengths, weakness, opportunities and threats (SWOT) of Indian Pharm.D are critically and scientifically analysed and evaluated in this study paper. Key Words.:- Doctor of Pharmacy, Pharm.D, Indian Pharm.D education, Pharm.D critical study.Download PDF


Title: EFFECT OF NUTRACEUTICAL ON HUMAN HEALTH: A REVIEW
Author: Kalita Ramen, Das Trishna
Keyword: Nutraceutical, Functional food, Classification, Health benefit.
Page No: 48-57
Abstract: Nutraceuticals are food product that provides health as well as medical benefits; including the prevention and treatment of disease. A few no of nutraceuticals are being used as pharmaceutical and a number of other being used by the general public as self-medication. Such products may range from dietary supplements to genetically engineered foods, herbal products and processed foods. Clinical research on nutraceutical product is going on for integrating and assessing information. Nutraceuticals are present in most of the food ingredients with varying concentration .Concentration, time and duration of supply of nutraceuticals influence human health.Manipulating the foods, the concentration of active ingredients can be increased.Diet rich in nutraceuticals along with regular exercise, stress reduction and maintenance of healthy body weight will maximise health and reduce disease risk. Keyword: Nutraceutical, Functional food, Classification, Health benefit. Download PDF


Title: IMPORTANCE OF HERBAL MEDICINE IN TREATMENT OF DENGUE FEVER – REVIEW
Author: Addepalli Sandhya, Harika Balya, S.Shanmuganathan
Keyword: Dengue, herbs, therapeutic efficacy
Page No: 58-63
Abstract: Dengue Fever is a disease caused by a family of viruses that are transmitted by mosquitoes. The “dengue triad” is a characteristic which include symptoms like severe muscle and joint pain, lymphade nopathy and bleeding gums. According to WHO, it estimates that approximately 22,000 deaths occurs annually, mainly among children. Indian medicinal herbs are gaining so much importance now-a-days due to their less side effects and more therapeutic efficacy and also used in the treatment of ailments such as asthma, fever, cough malaria etc. In our present review the use of natural home remedies using medicinal herbs such as Neem, Tulasi, Hogweed and Coriander etc for the treatment of dengue fever has been discussed. Keywords: Dengue, herbs, therapeutic efficacy Download PDF


Title: FORMULATION & ANALYSIS OF LEVOFLOXACIN HEMIHYDRATE BY RP-HPLC IN BULK & TABLET DOSAGE FORM
Author: Dey Bivha, Mahapatra Sujata, Tiwari Prabhakar
Keyword: Antibiotics, Fluoroquinolone, High Performance Liquid Chromatography, Levofloxacin, Quantitative analysis, Validation studies.
Page No: 64-77
Abstract: Abstract Objective(s) Developed and validate analytical method for quantitative determination of Levofloxacin tablet formulation. It was prepared by wet granulation method and evaluated by various p hysicochemical parameters. Analytical method was validating in different parameters like specificity, accuracy, linearity, precision, range, detection limit, robustness. Study in reversed phase high performance liquid chromatographic (RP-HPLC) method for the quantitative determination of Levofloxacin in bulk tablet and finished tablet. Materials and Methods: A shim-pack CLC-ODS column(25cm X 4.6mm, 5µm) and a mobile phase constituted of Buffer (850 ml of 0.05 (M) Citric acid monohydrate and 10 ml of 1 (M) ammonium acetate) :Acetonitirile (150) were used. The flow rate was 1 ml/min and the analyses performed using ultraviolet (UV) detector at a wavelength of 293 nm using levofloxacin Pure drug as an internal standard. Separation was completed within 15 to 17 min. Results: The developed method showed good resolution between formulated tablet and standard drug. The standard solution of Levofloxacin was prepared to obtain the concentration of about 100 ?g /mL, Six (6) replicate injection of homogeneous sample, their % RSD was 0.07 % & test solution was 0.17 %. The assay of Levofloxacin Tablet Obtained by Different Chemist, Date & Column 97.56 %, 98.69 % & 98.24 % respectively. The assay of different flow rate and temperature was 98.07% and 98.12% respectively. The prepared tablet was studied for its stability test for 24 hrs at preselected time interval like initial, 4 hrs, 8 hrs,12 hrs and 24 hours. Their assay % result was obtained 98.62, 98.54, 97.60, 98.50 and 98.41 % respectively. Conclusion: The method is linear, quantitative, reproducible and could be used as a more convenient, efficient and economical method for the trace analysis of drug in raw material and tablets. Keywords: Antibiotics, Fluoroquinolone, High Performance Liquid Chromatography, Levofloxacin, Quantitative analysis, Validation studies. Download PDF