ARCHIVES(Journal of Harmonized Research in Pharmacy)


Title: EVALUATION OF HAIR GROWTH ACTIVITY OF PUERARIA TUBEROSA BY USING STRESS INDUCED HAIR LOSS
Author: Monesh Likhitkar*and Milind Pande
Keyword: Isoflavonoid, Phytosterole, Pueraria tuberosa, Lymphocyte count, Hair density.
Page No: 01-06
Abstract: Background: We previously demonstrated that stress induce alopecia in mice. This effect was prevented by the oral administration of Pueraria tuberosa alcoholic extract, an isoflavone and phytosterol e containing plants which prevent hair loss alopecia condition. Objectives: The present study aimed at hair growth activity of Pueraria tuberosa by Using Stress Induced Stress induced alopecia and modulating the mechanisms responsible for this condition. Methods: 4 Group of mice were exposed whole-body to sonic stress. Groups of mice received the Pueraria tuberosa extract and standard Minoxidile 5% solution administered orally to elucidate the biological activity in vivo. Occurrence of alopecia was evaluated for up to 21 days of exposure, and the hair density, hair population, lymphocyte count and testosterone level in peripheral blood was investigated. Results: The hair density and hair population decrease and lymphocyte count was increased by stress; irrespective of treatment with ultrasonic sound. Test drug extract show decreases the lymphocyte count as compared to increased count in toxic group. Conclusions: Decrease in lymphocyte count in case of stress is a good sign of preventing hair loss due to the presence of isoflavonoid , protein and amino acid, insulin growth factor and may be due to increase blood circulation which promote hair growth in humans. Keywords: Isoflavonoid, Phytosterole, Pueraria tuberosa, Lymphocyte count, Hair density. Download PDF


Title: PHARMACOLOGICAL EVALUATION OF PLUMBAGO ZEYLANCIA LEAF EXTRACTS FOR ANXIOLYTIC ACTIVITY BY USING OPEN FIELD TEST
Author: N. Naga Amrutha*, T. Hemalatha, M.V. Anjali, D. Eswar Tony, Rama Rao Nadendla
Keyword: Anxiolytic activity, Plumbago zeylanica, stress, inferiority, backward.
Page No: 07-10
Abstract: Treatment for various problems through herbal medicines is a traditional system being practiced for thousands of years. As all can obey the practice due to fewer side effects, considerable research o n pharmacognosy, phytochemistry, pharmacology and clinical therapeutics has been carried out tremendously. Coming to the current research, on anti-anxiety or anxiolytic activity, most of the people in now a day have got feared in the present society due to many circumstances like stress, inferiority, backward in the hype areas in their own field. For this our basic research started with safe herbal extracts by using mice as experimental animals which are exposed on open field apparatus for evaluating anxiolytic activity. The current research got a good response as this procedure was very easy and fast for the evaluation. Download PDF


Title: QUERCETIN- A FLAVONOID IDENTIFIED IN BRYOPHYTE
Author: Dr.Deepti Suhalka
Keyword: Secondary metabolites, rutin, quercetin, antifungal
Page No: 11-15
Abstract: Bryophytes the plant amphibians contain numerous potential compounds, including oligosaccharides, sugar alcohols, amino acids, fatty acids, aliphatic compounds, aromatic and phenolic substances etc. These plants show fragrant odours and intensely hot and bitter taste as well as diuretic and anticancer activity. A number of bryophytes have been used as medicinal plants to treat burns, bruises, external wound. Traditionally the bryophytes possess some bioactive compounds and used throughout the world as drugs. These plants produce a high diversity of natural products or secondary metabolites with a prominent function in the protection against microbial pathogens. Secondary metabolites, including terpenes, phenolics and nitrogen (N) and sulphur (S) containing compounds These plants also offer a rich source of rare and structurally unique molecules and can serve as a reserve of potentially antifungal compounds for further development as pharmaceuticals. Biological control of plant diseases would help in preventing increase of pathogen. Among secondary metabolites flavonoids are a group of polyphenolic compounds, which are widely distributed throughout the plant kingdom. Flavonoids like Rutin and quercetin possess many biochemical effects. An HPLC method was developed for the estimation of quercetin from methanol extract of bryophyte. This review presents an overview about presence of some secondary metabolites in bryophyte extract and their antimicrobial activity. Keywords: Secondary metabolites, rutin, quercetin, antifungal Download PDF


Title: PHYTOCHEMICAL, CYTOTOXIC INVESTIGATION AND MOLECULAR DOCKING STUDIES OF CYTISUS SCOPARIUS Link FOR ITS DUAL HUMAN TOPO POISONING (I & II) ACTIVITY.
Author: M. V. N. L. Chaitanya*, S. P. Dhanabal, S. Jubie & M.Pavithra
Keyword: Phenolics, flavanoids and saponins.
Page No: 16-35
Abstract: Highlights: • The aerial parts of weed plant Cytisus scoparius (L.)Links have good amounts of phenolics, flavanoids and saponins. • A novel phenolic compound (1E)-1-hydroxyprop-1-en-2-yl (2E)-3-(4-h ydroxy-3-methoxyphenyl)prop-2-enoate (CH2) was isolated and characterized first time from this plant using Flash chromatography. • First time, the total saponin fraction was isolated and characterized by LCMS finger printing and found to be have ruscogenin as one of the saponin • The total saponin fractions and isolated compound showed significant antioxidant and anticancer properties. • To study their mechanism of action for cytotoxicity these molecules have been docked into the crystal structure of Topo –I and Topo-II enzymes usingSchrödinger suite, 2014-3. COMPOUND SELECTED FOR THE PRESENT STUDY S.NO Name of the compounds (molecular weight) Structure 1. (1E)-1-hydroxyprop-1-en-2-yl (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate (CH2) ( 250.2) 2. Ruscogenin (430.6) • Download PDF


Title: ISOLATION AND INVESTIGATION OF ANTIMICROBIAL ACTIVITY OF THE LEAVES OF THEA SINENSIS
Author: Jiten Mishra*, Sanjay Kumar Nayak and Shudhir Kumar Sahoo
Keyword: Thea sinesis, ZOI, MIC
Page No: 36-39
Abstract: In the present study the ethanolic extract and isolated caffeine obtained from the Thea sinesis were tested for antibacterial activity against 13 Bacterial strains both gram positive and gram negativ e by determining minimum inhibitory concentration (MIC) and zone of inhibition (ZOI). MIC values were compared with standard chloramphenicol in concentrations of 30 & 100 µg/ml. The result of MIC & ZOI and its competition with standard antibiotics it evident that the ethanolic extract is active against both gram positive and gram negative bacteria. Further the isolated caffeine was characterized by different analytical techniques like TLC, UV-VIS spectroscopy & HPLC and compared with the slandered. Key Words: - Thea sinesis, ZOI, MIC Download PDF


Title: MONITORING OF MARKETED PORK OF ASSAM AND MEGHALAYA FOR CIPROFLOXACIN RESIDUES
Author: D.C. Roy, R. Gogoi and S.K. Laskar
Keyword: Ciprofloxacin, Monitoring, Pork, Residues, UHPLC
Page No: 40-43
Abstract: The study was undertaken to monitor marketed pork of Assam and its neighboring state Meghalaya for Ciprofloxacin residues using Ultra High Performance Liquid Chromatography (UHPLC). 300 samples of ma rketed pork of Assam and Meghalaya were collected for the study. The samples after collection were preserved at -20°C. Recoveries of ciprofloxacin in pork ranged from 88-98%. Out of the tested samples, 18 samples were detected to be positive for trace residues of Ciprofloxacin which were well below the Maximum Residue Limit (MRL) value. Keywords: Ciprofloxacin, Monitoring, Pork, Residues, UHPLC Download PDF


Title: FORMULATION AND EVALUATION OF MODIFIED DISINTEGRATING SUSTAINED RELEASE TABLETS OF ACECLOFENAC
Author: Lukkad Harish R* and Oswal Rajesh
Keyword: Oral drug delivery, Sustained release (SR) tablets, Fast disintegrating tablets (FDT), Microspheres, Superdisintegrants
Page No: 44-51
Abstract: Oral drug delivery is the largest and the oldest segment of the total drug delivery market. It is the fastest growing and most preferred route for drug administration. In oral drug delivery, the sust ained release (SR) tablets maintains the desired drug concentration for prolong period of time, reduced 'see- saw' fluctuation, reduced total dose, improved efficiency in treatment. But many patients like paediatric, geriatric and also patients may have difficulty in swallowing (Dysphagia) find it difficult to swallow tablets and thus do not comply with prescription. This problem is overcome by formulating and developing modified disintegrating sustained release tablets. In this case, first microspheres of the drug are formulated by using any suitable technique. And then optimized microspheres formulation is further formulated in to the fast disintegrating tablets (FDT) by using superdisintegrants. So that after taking such a tablets, the tablet only disintegrates into the mouth then microspheres are separated and ingestion of such microspheres starts releasing drug for prolonged period of time. This concept fulfills both the advantages of sustained release and fast disintegrating tablets. Keywords: Oral drug delivery, Sustained release (SR) tablets, Fast disintegrating tablets (FDT), Microspheres, Superdisintegrants Download PDF


Title: DESIGNING AND PERFORMANCE EVALUATION OF CLARITHROMYCIN BIOADHESIVE TABLET
Author: Atul Gunjal*, Santosh Ghule, Gali Vidyasagar
Keyword: Bioadhesive, Controlled release, Granulation, Compression, Release kinetic
Page No: 52-59
Abstract: The objective of present research work was to design and evaluate bioadhesive tablets of clarithromycin. Tablets were prepared by wet granulation technique and evaluated for pre-compression as well a s post compression parameters. Different formulation batches (C1-C6) were prepared and evaluated for characteristics parameters like hardness, friability, drug content, weight variation etc. Tablets were also evaluated for bioadhesive strength, and in vitro release profile. Further data obtained from in vitro release study was subjected to release kinetics. Results suggested that batch C1 showed controlled release with excellent bioadhesive strength. Key words: Bioadhesive, Controlled release, Granulation, Compression, Release kinetic Download PDF


Title: EVALUATION OF PSYCHOTROPIC EFFECT OF ZIZIPHUS MAURITIANA LEAVE EXTRACT
Author: Ganesh N. Sharma
Keyword: Ziziphus mauritiana, CNS depression, flavonoids, muscle relaxation
Page No: 60-66
Abstract: The Ziziphus mauritiana fruit are edible in nature and are being consumed worldwide. Researchers have isolated and identified a number of phytoconstituents within the different parts of the plant. At the same time many pharmacological values of the plant have also been reported, and these has been correlated with its phytoconstituents. The aim of present study is to evaluate Ziziphus mauritiana leaves for their effect on central nervous system. Materials and Methods: The aqueous and methanol extract was prepared and were analysed qualitatively for present phytoconstituents, followed by estimation of phenol and flavonoid contents. Subsequently; the extracts were evaluated for their effect on CNS using different preclinical screening models. Results: Total phenol contents in methanol and aqueous extract was found 42.13 and 13.32 (µg/mg of GAE), respectively. Total flavonoid contents were 7.25 and 4.97 (mg of rutin/100g extract) in respective extracts. The animal treated with extracts shown significant reduction in locomotion, muscle griping power, antianxiety, and antiepileptic property as compared to control group animals. Conclusion: The study results suggest that the leave extract of Ziziphus mauritiana possesses remarkable CNS depressant property. It was also postulated that the inhibition in CNS activity might be due to the present phenolic compounds; and more appropriately the flavonoid moieties. Keywords: Ziziphus mauritiana, CNS depression, flavonoids, muscle relaxation Download PDF


Title: ETHNOMEDICINAL AND TRADITIONAL USES OF ELAEOCARPUS GANITRUS (RUDRAKSHA)
Author: Harish Gupta, Shubhangi Dwivedi, Abhijit Dubey and Peeyush Jaiswal
Keyword: Elaeocarpus ganitrus, Rudraksha, Mukhi, Elaeocarpaceae, Phytochemistry, Pharmacological.
Page No: 67-77
Abstract: In the present review, we integrate the botanical, phytochemical, ethnomedicinal, pharmaco-logical information on Elaeocarpus ganitrus belongs to family Elaeocarpaceae. The ancient Yogi's extensivel y explained about useful healing powers of Rudraksha bead. When time is passed over the year people gained Rudraksh beads as their healing and also as a fashion accessory. After this in the recent year scientific studies have been taken in India to determine the degree and mechanism of the healing powers of Rudraksha. In prestigious Indian Institute of Technology, Banaras, India, Dr. Subhas Rai published one of the best known research papers about Rudraksh. Some researcher confirmed that Rudraksha beads have dominant electromagnetic, paramagnetic and inductive properties. These properties describe the healing powers of Rudraksha bead. Furthermore it is retrieve to exhibit multifarious pharmacological activities like anti-inflammatory, analgesic, hypoglycemic, antiulcerogenic and very high antimicrobial activity. This review provides a scientific basis for pharmacological /medicinal properties and therapeutic uses of Elaeocarpus ganitrus. Keywords: Elaeocarpus ganitrus, Rudraksha, Mukhi, Elaeocarpaceae, Phytochemistry, Pharmacological. Download PDF


Title: EVALUATION OF ANTIDEPRESSANT ACTIVITY OF HYPERICUM HIRCINUM
Author: Mathew George*, Lincy Joseph, Ashitha.K.S
Keyword: Antidepressant activity, Hypericum hircinum, rat.
Page No: 78-82
Abstract: Objective: To study the effect of ethanol: acetone extract of Hypericum hircinum have antidepressant activity on Wistar albino rat. Methods: Whole plant was collected and extracted using ethanol: ac etone by maceration. The crude extracts were screened for the biological activity through the assay behavioral study. Antidepressant activity evaluated by using behavioral methods such as ketamine induced sleep, tail suspension method. Result: Hydro alcoholic extract of have significant antidepressant effect on rat. Conclusion: The result indicates that whole plant hydroalcoholic extract was an antidepressant agent. Key words: Antidepressant activity, Hypericum hircinum, rat. Download PDF