ARCHIVES(Journal of Harmonized Research in Pharmacy)


Title: EVALUATION OF ANTIPYRETIC ACTIVITY OF VARIOUS EXTRACTS OF LEAVES OF MENTHA ARVENSIS IN RAT
Author: Raghvendra Mishra, D.N.Jhade, Sunil Shah, Chandra Kishor Tyagi, Mithun Kumar Verma
Keyword: Mentha arvensis Linn, Brewer’syeast, antipyretic, phytochemical analysis, paracetamol.
Page No: 122-138
Abstract: Mentha arvensis Linn. is described as a antipyretic in Ayurveda, the Indian system of Medicine. There is no reaserch reports in themodern literature regarding its usefulness as a antipyretic agent. T he present studywas carried out to evaluate the antipyretic activity of extracts of leaves of Mentha arvensis Linn. Brewer’syeastinduced pyrexia in male Albino rats. Materials and Methods: Different leaf extracts of Mentha arvensis Linn were subjected to preliminary phytochemical analysis and evaluated for their antipyretic potential employing yeast induced pyrexia in Albino rats. Results: The ethanolic, chloroform and petroleum ether leaf extract exhibited maximum antipyretic activity (P<0.001) at doses of 250 and 500 mg/kg-1b.w. p.o. in a dose dependent manner and the antipyretic activity was comparable to that of reference standard paracetamol and aqueous extract did not showed any significant antipyretic activity. Phytochemical analysis revealed presence of flavonoid, triterpenoids, sterols and alkaloids, which have been known for their antipyretic activities. Conclusion: The Ethanolic extract of Mentha arvensis Linn of leaves. Possessed antipyretic activity and this may be due to the presence of flavonoids and triterpenoids, because as per literature they showed good antipyretic activity. However, further molecular level word is required to determine the exact mechanism involved in antipyretic activity of Mentha arvensis Linn. leaves. Key words: Mentha arvensis Linn, Brewer’syeast, antipyretic, phytochemical analysis, paracetamol. Download PDF


Title: PREPARATION AND EVALUATION OF POROUS PELLETS LOADED WITH INDOMETHACIN FOR CONTROLLED RELEASE
Author: Sandeep Kumar Jaiswal, D. N. Jhade, Sunil Kumar Shah, Chandra Kishor Tyagi
Keyword: Indomethacin Pellets, Controlled release, Extrusion/Spheronisation.
Page No: 139-155
Abstract: The aim of the present study was to prepare and evaluate microporous pellets loaded with Indomethacin is an oral Non-steroidal anti-inflammatory drug, indicated for the treatment of rheumatoid art hritis. Indomethacin is indicated for the treatment of rheumatoid arthritis, alone or in combination with other NSAID’S drugs. Indomethacin is also indicated for the treatment of ankylosing, spondylitis, osteoarthritis. The frequent adverse effects of NSAIDs like Indomethacin are gastro-intestinal disturbances. Enteric coated formulations minimize these symptoms. Multi-particulate system such as porous pellets disperse freely in the gastro-intestinal tract, contributes to maximum drug absorption, reduced peak plasma fluctuations and have less side effects. Porous pellets were prepared by extrusion /spheronization method. Keywords: Indomethacin Pellets, Controlled release, Extrusion/Spheronisation. Download PDF


Title: DETERMINATION OF ANTIBACTERIAL ACTIVITIES AND MODE OF ACTION OF DIFFERENT FRACTIONS OF ACACIA ARABICA LAM
Author: Rajbhan Kushwaha, D.N. Jhade, Sunil Shah, Chandra Kishor Tyagi, Mithun Kumar Verma
Keyword: Acacia arabica Lam Bacillus subtilis, Staphylococcusaureu, Antibacterial activity
Page No: 156-164
Abstract: Micro organisms are subject to either favorable or antagonistic reactions owing to the presence of other living organisms. To avoid the side effects of synthetic drug in the recent years herbal drugs and their formulations are largely in use. Here we also used the fractions of extract of Acacia Arabica lam for the antimicrobial study by using the antibiotic as a standard. So; it was proposed to attempt antimicrobial study and Minimum Inhibitory concentration of fraction of bark of acacia Arabica lam. It is evident from literature survey that no antimicrobial method was mention on the various fractions of Acacia arabica bark as well as its mode of action. In present study, an attempt has been made to derive the antimicrobial action and its mode of action by using different strains of bacteria on the different fractions of Acacia Arabica The development of antimicrobial drugs represents one of the most important advances in therapeutics, both in the control or cure of serious infections and in the prevention and treatment of infectious complications of other therapeutic modalities such as cancer chemotherapy and surgery. Antimicrobial agents are frequently used before the pathogen responsible for a particular illness or the susceptibility to a particular antimicrobial agent is known. This use of antimicrobial agents is called empiric therapy and is based on experience with a particular clinical entity. The usual justification for empiric therapy is the hope that early intervention will improve the outcome; in the best cases, this has been established by placebo-controlled, double-blind prospective clinical trials. Key words: Acacia arabica Lam Bacillus subtilis, Staphylococcusaureu, Antibacterial activity Download PDF


Title: DEVELOPMENT OF QUALITY STANDARDS OF PHYLLANTHUS AMARUS LEAVES
Author: Om Prakash Agrawal*, Girendra Gautam
Keyword: Phyllanthus amarus, HPTLC, Pharmacognostical standardization.
Page No: 165-170
Abstract: Phyllanthus amarus (Euphorbiaceae) is an important plant of Indian Ayurvedic system of medicine which is used in the problems of stomach, genitourinary system, liver, kidney and spleen. Morphological examination showed that leaves are long, palmate shaped and dark green in color. The leaves powder had characteristic odor and taste. Transverse section of leaves showed upper and lower epidermis with covering and glandular trichomes and midrib showed arc shaped vascular bundle. Successive extractive value was highest (23.606%) in case of aqueous extract. Mean ash values (%) were 23.04 (total), 6.48 (acid insoluble) and 12.69 (water soluble). Loss on drying was 5.9107%. Resin content was found 1.33%. Phytochemical screening of leaves powder showed the presence of carbohydrates, phenolic compounds, flavonoids, steroids, tannin, resin and acidic compounds. Further work is needed to isolate, characterize and quantify active constituents present in the leaves of Phyllanthus amarus by sophisticated techniques. Keywords: Phyllanthus amarus, HPTLC, Pharmacognostical standardization. Download PDF


Title: ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS OF Garcinia cambogia AS MARKERS
Author: Manoj Kumar Kamshetti, Sunita Jhade, Pushpendra Sharma, Deenanath Jhade
Keyword: Garcinia cambogia, (-)-Hydroxy Citric Acid, HPLC.
Page No: 171-177
Abstract: Background and Objectives: Garcinia cambogia (Guttiferae), generally known as Malabar tamarind is a dried fruit rind commonly found in Western ghats of south India. It extends from Konkan southward to Travancore and in the forests of Nilgiris. It is highly regarded for their medicinal properties in the indigenous systems of medicine. The present study deals with the isolation and characterization of the chemical constituents of Garcinia cambogia. Methodology: Authenticated aqueous extract of Garcinia cambogia is collected from Pharmed Medicare, Bangalore. Phytochemical screening of extract has been done. Isolation of phytoconstituents by column chromatography and/or alternate method of isolation was carried out with aqueous extract. The isolated compound was identified by comparing the HPLC fingerprint of the sample with the standard chromatogram of (-)-HCA. Results: One compound has been isolated and estimated as (-)-Hydroxy Citric Acid by comparing the HPLC data of sample and standard. Conclusion: The isolation procedure used is suitable to isolate the marker constituent from aq extract of Garcinia cambogia. Keywords: Garcinia cambogia, (-)-Hydroxy Citric Acid, HPLC. . Download PDF


Title: STUDY OF ANAEMIC STATUS IN GIRL STUDENTS OF SIDDESHWAR VIDHYALAYA, KHASGI (MS)
Author: Shivakumar S. Ladde*, Dinesh S. Gujrathi, Dharashive V. M., Nagoba S. N.
Keyword: Anaemia, Haemoglobin, Adolescents, Khasgi.
Page No: 178-182
Abstract: Aim: To examine the prevalence of anaemia in Girl Students at Siddeshwar Vidhyalaya, Khasgi Tq-Omerga, Dist Osmanabad, Maharashtra. Material and Methods: The present study included 637 adolescent gir ls selected from Siddeshwar Vidhyalaya, Khasgi Tq-Omerga, Dist Osmanabad, Maharashtra and the study included the all adolescent girls are categorised into two groups i.e. Age Between 10-13yrs (5-8th Class) and Age Between 14-18yrs (9-12th Class). The Heamoglobin is estimated by Sahli’s/acid hematin Method. The severity of anaemia was categorized as severe (<7 g/dl), moderate (7-9.9 g/dl) and mild (10-11.9 g/ dl). Result: Present research findings revealed that out of 637 studied subjects, only 55.88 per cent adolescent girls were having normal haemoglobin value whereas 44.12 per cent were affected with various grades of anaemia i.e. 17.26per cent moderately anaemic and 26.84 per cent mildly anaemic. Conclusion: In the present study it conclude that anaemia in girls dueto malnutrition hence we advised to school health authority should impart nutrition education among school adolescents girls to prevent the nutrition anemia. Keywords: Anaemia, Haemoglobin, Adolescents, Khasgi. Download PDF