ARCHIVES(Journal of Harmonized Research in Applied Science)


Title: ETHNOBOTANICAL STUDY OF MULTIPURPOSE AND FRAGRANT PLANTS ON THE FARMING SITES IN GOZAMIN DISTRICT, AMAHARA REGION, ETHIOPIA.
Author: Haimanot Reta, Zemede Asfaw, Ensermu Kelbessa
Keyword: Multipurpose plant, farming zone, gozamin district, fragrant plant
Page No: 94-107
Abstract: An ethnobotanical study aimed at documenting indigenous knowledge on multipurpose and fragrant plants maintained in the farming sites was carried out in Gozamin District located in western Ethiopia. Six kebeles were selected from the altitude range between 1500-3200 m a s l. A total of 45 plant species were collected from five farming zones centering the house and moving away from it. Sixty household heads were systematically selected from 120 household heads having well established homegardens and farm fields. The ethonobotanocal data were collected through interview, guided field walk, group discussion, semi-structured interview and participant observation. Ten key informants were involved in the interview to recover more in-depth ethnobotanical information focusing on multiple purposes and for fragrance. The market utility values of desired fragrant plants were further analyzed by paired comparison and 10 multipurpose plants were further evaluated by using direct matrix ranking. The plants were grouped as multipurpose plants (39), both multipurpose and fragrant (1), fragrant and medicinal plant (4), and only fragrant plant (1). It was observed that farmers evaluate the plant based on different criteria acquired through accumulated experience in a different way than people trained in modern agriculture. Evaluation of the plants by farmers of the study area using different criteria has resulted in some species tolerated, others managed and/or cultivated by households. Farmers must be consulted before deciding on the usefulness of any plant in their environment. Key words: Multipurpose plant, farming zone, gozamin district, fragrant plant Download PDF


Title: EVALUATION OF THE VARIETAL RESISTANCE OF COWPEA [VIGNA UNGUICULATA (L.) WALP.] TO THE APHIDS (APHIS CRACCIVORA KOCH) IN THE FAR NORTH OF CAMEROON.
Author: P Kosma, MD Magoudji, G Sobda
Keyword: Vigna unguiculata, microsatellite markers, Aphis craccivora, varietal resistance.
Page No: 108-121
Abstract: Cowpea is one of the food products that ensure food security in the Far North Region of Cameroon. However, production yields remain low due to many parasitic constraints, due particularly to Aphis cr accivora. The commonly used control strategy is based mainly on chemicals whose usage is polluting, onerous and restrictive. This work was undertaken to assess the resistance of 10 varieties of cowpea A. craccivora, pest of cowpea in the Far North Region of Cameroon. For this purpose, after infestation of the seedlings by 5 aphids nymphs each, stem height, the number, length and width of primary leaves on the one hand and the number and the damage of the other aphids were evaluated. Thereafter, 40 pairs of primers were tested for identification of closely linked microsatellites resistance gene to aphids. The results obtained show that the varieties NGT115, SARC-1-57-2, KVX-165-14-1, LORI, IT97K-556-6 IITA, B-301 and APAGBALA are tolerant. The varieties KVX-295-2-124-99 and BR1 are sensitive and variety VYA is very sensitive.Two pairs of primers CP51 / CP52 and CP53 / CP54 identified morphic single markers which can therefore not be used for the detection of the gene for resistance to aphids. Other primers have not identified anything. These results demonstrate the need to find sources of resistance in the native material. They show the need to further look for polymorphic markers closely associated with resistance genes to aphids in order to integrate the marker-assisted breeding programs. Key words: Vigna unguiculata, microsatellite markers, Aphis craccivora, varietal resistance. Download PDF


Title: BIOLOGICAL MANAGEMENT OF SOUTH AMERICAN TOMATO MOTH (TUTA ABSOLUTA MEYRICK) IN ETHIOPIA
Author: Feyissa Begna
Keyword: Tomato, Bacillus thuringiensis, coragen, Tuta absoluta, miya
Page No: 122-131
Abstract: South American tomato moth (Tuta absoluta Meyrick) is one of the production constraints of Solanaceous crop of tomato (Lycopersicon esculentum Mill.). The trial was conducted using irrigation at Melk assa Agricultural Resarch Center (MARC) during 2014 off season to determine the optimum rate and frequency, and evaluate the efficacy of Bacillus thuringiensis var kurstaki against T. absoluta. The miya variety was used for this experiment. The experiment was laid out in RCBD with three replications. Bacillus thuringiensis was tested at 1 and 2 kg/ha in 7, days of foliage application in addition coragen as the standard check and the untreated check was included for comparison. The different doses of Bacillus thuringiensis were no variation on egg density and agronomic characters such as plant height, number of flower and fruit per cluster, and fruit set percentage. All treatments foliar application minimizing the leaf damaged score compared with untreated check. Coragen and 2kg/ha of B.t in weekly application were gave the maximum marketable yield, lowest fruit infestation percentage and fruit holes with T.absoluta and minimizing yield loss. Bacillus thuringiensis var kurstaki which are medium efficacy in controlling T.absoluta after the study compared with the standards check. It is an important component of integrated pest management so more extensive studies for integrating of Bacillus thuringiensis with botanicals and biological control methods including predator and parasitoids of control measures against T.absoluta would contribute to sustainable tomato production in Ethiopia. Key words: Tomato, Bacillus thuringiensis, coragen, Tuta absoluta, miya Download PDF


Title: ANTI-ANEMIC ACTIVITY OF HYDRO-ALCOHOLIC LEAF EXTRACT OF TAMARINDUS INDICA IN PHENYLHYDRAZINE INDUCED ANEMIC RATS
Author: Joshi Ankur*, Gupta Deepanshu, Soni Priyanka, Malviya Sapna, Jhade Deenanath & Kharia Anil
Keyword: Anemia, Anti-anemic activity, Tamarindus indica and B-complex vitamin
Page No: 132-135
Abstract: The aim of the current study is to test the anti-anemic activity of hydro-alcoholic leaf extract of Tamarindus indica against phenylhydrazine induced anemia in rats. Phenylhydrazine (60mg/kg) was adm inistered intraperitoneally for two days to induce anemia in rats. The animals were divided in to four group containing six animals each. Group I served as normal control, Group II as anemic control, Group III as reference control administered with B-complex vitamin and Group IV animals were treated with 200mg/kg, of hydro-alcoholic leaf extract of Tamarindus indica. All the test drugs were administered once daily for twenty eight days through oral route. On twenty ninth days blood was withdrawn, through tail puncture under phenobarbitone anesthesia and subjected to the estimation of erythrocyte, hemoglobin and percentage Hematocrit. both the hydro-alcoholic leaf extract of Tamarindus indica and vitamin B12 significantly increased the erythrocyte, hemoglobin and Hematocrit levels that conclude that, Tamarindus indica leaf extract exhibits anti-anemic activity. Keywords: Anemia, Anti-anemic activity, Tamarindus indica and B-complex vitamin Download PDF


Title: STUDY OF PLANT GROWTH ACTIVITY OF 2-METHOXY-6-{[2-(2-METHOXY-PHENOXY)-ETHYLIMINO]-METHYL}-PHENOL AND ITS TRANSITION METAL COMPLEXES ON TRIGONELLA FOENUM-GRAECUM, TRITICUM AESTIVUM AND BRASSICA NIGRA.
Author: Sheetal V. Palande* and Dr. Deelip K. Swamy
Keyword: Schiff bases, Metal Complexes, Plant growth studies, Standard Blotter method.
Page No: 136-140
Abstract: Coordination complexes of transition metals with Schiff base ligand were synthesized. The characterization of these complexes were elucidated by physical parameters and spectral analysis namely colou r, melting point, IR, NMR, UV, Magnetic measurements, TGA and ESR studies.. Plant growth regulating activity on seeds of Trigonella foenum-graecum (Methi), Triticum aestivum (wheat) and Brassica nigra (black mustard) has been studied using standard Blotter method for evaluation of inhibitory or stimulatory effects of the synthesized compounds. The plant growth analysis was decided by measurement of parameters like percentage of germination, seedling height, shoot length, root length, root/shoot ratio and vigor index. The values of these parameters have been used to make a conclusion about plant growth regulating activity of ligand and its complexes Keywords: Schiff bases, Metal Complexes, Plant growth studies, Standard Blotter method. Download PDF


Title: SIMULTANEOUS ESTIMATION OF ABACAVIR, LAMIVUDINE AND DIDANOSINE TRIPHOSPHATE IN TABLET DOSAGE FORMULATION BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author: Rajeev K. Singh* and Dr. Pushpendra Sharma
Keyword: Method Development, Validation, HIV, Anti-retroviral, Simultaneous estimation.
Page No: 141-145
Abstract: A new, simple, rapid, selective, precise and accurate isocratic reversed-phase high performance liquid chromatography assay has been developed for simultaneous estimation of Abacavir Sulphate, Lamivu dine and Didnosine Triphosphate in a marketed tablet formulation. The separation was achieved by using C-18 column (Phenomenex, 250×4.6mm i.d.) particle size 5µ coupled with a guard column of same material, in mobile phase methanol: Acetonitrile: Ortho phosphoric acid in ratio of 75:25:0.2 respectively with maintain of pH 4.5 0.1 at 1.0 mL/min. Selected wavelength at 245 nm and sample volume injected 20 µL at ambient temperature. Total run time was 10 min; abacavirsulphate, lamivudine and didanosine triphosphate were eluted with retention time of 2.25, 3.55 and 5.50 minutes respectively. The developed method was validated as per ICH guidelines. Validation revealed that method is specific, rapid, accurate, precise, reliable and reproducible. Key-Word: Method Development, Validation, HIV, Anti-retroviral, Simultaneous estimation. Download PDF