IN PRESS


Title: EVALUATION OF ANTIMICROBIAL PROPERTIES, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS OF PLANT EXTRACTS LAVANDULA STOECHAS LINN
Author: Brijesh Sirohi and Rakesh Sagar
Keyword: Lavandula Stoechas Linn, Phytochemical Screening, Antimicrobial, Total Flavonoid, Total Phenolic content.
Page No: 6-11
DOI: 10.30876/JOHR.8.2.2019.06-11
Abstract: Lavandula Stoechas is a Plant of the Lamiaceae/Labiatae family. The aim of this study was to screen the Phytochemicals, to evaluate the total flavonoid and total Phenolic contents as well as an Antimi crobial activity of hydroalcoholic extract of Lavandula Stoechas. Plant material was extracted by using hydroalcoholic solvent for 2 h and repeated 3 times. Total flavonoid content was determined by aluminium chloride colorimetric assay on 420 nm. Total Phenolic content was determined with Folin-Ciocalteu 1:4 on 765 nm using microplate reader. Phytochemical screening showed that all of samples positively contain alkaloid, glycoside, flavonoid, phenolics, carbohydrate, diterpenes and saponin. Total flavonoid content was found to be 9.31(QEmg/100mg) where as total Phenols was found to be 5.52 (GAEmg/100mg). Which could be related to its higher Phenolic content. It can be hypothesised that the high contents of Phenolic compounds of Lavandula Stoechas indicated that these compounds contribute to the Antimicrobial activity and can be regarded as promising plant species for natural sources of Antimicrobial activity with potential value for treatment of many life threatening diseases.Download PDF


Title: KNOWLEDGE, ATTITUDE, AND PRACTICES ON ANTIBIOTIC RESISTANCE AMONG PHARMACISTS AT THE UNIVERSITY TEACHING HOSPITALS IN LUSAKA, ZAMBIA
Author: Steward Mudenda, Fred Fredrick Bangara, Jonathan Sitali, Michelo Banda
Keyword: Antibiotic resistance; Knowledge; Attitude; Practices; Pharmacists
Page No: 12-24
DOI: 10.30876/JOHR.8.2.2019.12-24
Abstract: Antibiotic resistance is a global public health problem that affects everyone. This study aimed to explore the knowledge, attitude, and practices on antibiotic resistance among pharmacists at the Univ ersity Teaching Hospitals in Lusaka, Zambia. This descriptive cross-sectional study was done among 46 randomly selected hospital pharmacists from November 2018 to February 2019. Data was analysed using Statistical Packaging for Social Sciences version 20. Statistical associations were done using Chi-square, Wilcoxon-Mann Whitney and McNemar-Bowker test. Statistical significance was conducted at 95% confidence level with a p<0.05 to indicate statistical significance. The majority of participants 24/46 (52%) were males while 22/46 (48%) were females (p=0.883). Majority of the participants were in the age group of 26-30 years old (p=0.010), the minimum age was 23 and maximum age was 47 years, mean age was 32.11 years, a standard deviation of 6.533, p=0.263. The majority of participants 28/46 (61%) were married (p=0.185), the majority of participants 24/46 (52%) had worked for a period of 1-5 years (p<0.001). All participants 46/46 (100%) were pharmacists (p<0.001) and were all Christians (p<0.001). Majority of the participants had adequate knowledge, positive attitude and good practices towards antibiotic resistance based on their responses to the questionnaire. Statistical tests showed no association between knowledge and attitude (p=0.693), knowledge and practices (p=0.409), and between attitude and practices (p=0.226). The majority of pharmacists at the University Teaching Hospital in Lusaka have good knowledge, positive attitude and good practices towards antibiotic resistance. Additionally, there was no association between knowledge and attitude (p=0.693), knowledge and practices (p=0.409), and attitude and practices (p=0.226). Keywords: Antibiotic resistance; Knowledge; Attitude; Practices; Pharmacists Download PDF