ARCHIVES(Journal of Harmonized Research in Pharmacy)

Author: Hemlata Rathore, Bhushan Hatwar & Shishupal Bodhankar
Keyword: Chlorophytum boriviliuanum, Antidiabetic & Antioxidant
Page No: 37-43
DOI: 10.30876/JOHR.8.3.2019.37-43
Abstract: Objective: To investigate the antidiabetic effect of aqueous extract of Chlorophytum borivilianum (safed musli) root. Methods: The collected roots were washed; shade dried and was pulverized with a me chanical pulverizer for the size reduction. It was then passed through mesh of size # 60 and the fine powder was collected and was used for the experiment for powder microscopy and preparation of extract. The root powder (1000 g) was subjected to cold maceration in 2 L of sterile distilled water for 48 hours at room temperature, filtered into a clean round bottom flask. The a- Glucosidase inhibitory activity was conducted where 100µl of a sample of different concentrations was incubated with 50µl a-glucosidase (1.0 U/ml) (from Saccharomyces cerevisiae) in phosphate buffer (0.1 M, pH 6.8) for 10 min at 37°C. The reaction was initiated by addition of 50 µl of substrate: 5 mM, p-nitrophenyl-a-D glucopyranoside in a 0.1 M phosphate buffer at pH 6.8. P-nitrophenol’s release kinetics. The a -Amylase Inhibition where 250µL of the extract (5mg/mL) was preincubated with 250µL of -amylase solution for 10min at 25°C in one set of tubes. In another set of tubes -amylase was preincubated with 250µL of phosphate buffer (pH 6.9). 250µL of starch solution at increasing concentrations (0.30–5.0mg/mL) was added to both sets of reaction mixtures to start the reaction. The mixture was then incubated for 10min at 25°C and then boiled for 5min after the addition of 500µL of DNS to stop the reaction. Results: In this study acarbose was also used as a standard drug for a-glucosidase inhibitor. Acarbose at a concentration of (100-1000 µg/ml) showed a-glucosidase inhibitory activity from 30. 31±0.1084to 84.05±0.2075% with an IC value 411.436567µg dry extract, & acarbose was also used as a standard drug for a amylase inhibitor. Acarbose at a concentration of (100-1000 µg/ml) showed a-glucosidase inhibitory activity from 23.33±0.1415 to 62.05±0.2725% with an IC value 236.774194µg dry extract. Conclusions: As a result, we found that the extract of C. boriviliamum have free radical scavenging activity and inhibitory activity against a-amylase and a-glucosidase and this therapeutic potentiality could be exploited in the management of post prandial hyperglycemia in the treatment of type 2 diabetes mellitus. Download PDF

Author: Dharna Singh, Ritesh Jain and Dr. Ankita Wal
Keyword: Ipomea hederacea, DPPH, ROS, Anti-oxidant
Page No: 44-49
Abstract: In present study, phytochemical and in-vitro antioxidant potential of Ipomea hederacea, a widely used medicinal plant, were investigated. Methanolic and aqueous extract of leaves and stem of Ipomea he deracea showed inhibition of scavenging of 1,1-diphenyl-2picrylhydrazyl (DPPH) radical and inhibition of reactive oxygen species (ROS) in vitro. Download PDF

Author: Kumar Ajay, Jitendra Bajaj*, Neeraj Upmanyu
Keyword: Liver, liver functions, liver diseases, liver treatments.
Page No: 50-54
Abstract: Liver is the most complex and important organs in the human body. A total loss of liver function leads to death within minutes. Liver is the largest gland in the body. It weighs about 1.5 kg (3 to 4 p ounds), lies immediately under the diaphragm, and occupies most of the right hypochondrium and part of the epigastrium. The common disorders of the liver include cirrhosis, viral hepatitis, alcoholic liver disease, hemochromatosis, and liver cancer. Liver transplantation is often the best option for either liver cancer or cirrhosis. There is an extreme shortage in the number of donor organs available, and there are restrictions on who can receive liver transplants. Due to these problems, alternatives are constantly being sought. Some of the primary areas of research involve gene therapy, xenotransplants, and bioartificial livers. Download PDF