Journal of Harmonized Research in Pharmacy

ANTICONVULSANT SCREENING OF HYDRO-ALCOHOLIC ROOT EXTRACT OF BERBERIS ARISTATA IN MICE

Abstract

Author(s): Chauhan S. Suchita and Sharma K. Hemant

To explore anti-convulsant potential of hydro-alcoholic extract of Berberis aristata (B. aristata) from pentylenetetrazole-induced and maximal electroshock (MES) seizure test. Material & Me thods: The test groups receive different doses (50, 100, 250 and 500 mg/kg) of the hydro-alcoholic extract; however positive control (standard) Phenobarbitone (20 mg/kg) using for Pentylenetetrazole-induced Seizure and Diazepam (1mg/kg) used as standard for maximal electroshock induced seizure. Swiss albino mice weigh about 20-25 g of either sex selected for the investigation. Results: Phytochemical investigation reveals that occurrence of alkaloids, flavonoids in the hydro-alcoholic root extract of Berberis aristata. Acute toxicity study shows no morality action up to 1000 mg/kg dose after 72 h observation. Hydro-alcoholic extract of B. aristata offered a concentration depending upon act of pentylenetetrazole-induced seizure (PTZ) and maximal electroshock induced seizure (MES). Conclusions: These finding suggest that hydro-alcoholic extract of B. aristata remarkable effect in the treatment in epilepsy. Key-words: Anti-convulsant, Berberis aristata, MES, Pentylenetetrazole, Diazepam, Epilepsy.