Journal of Harmonized Research in Pharmacy

PHYTOCHEMICAL ANALYSIS ON ASYSTASIA GANGETICA (L.) T. ANDERSON

Abstract

Author(s): Janakiraman N, Jasmin Jansi J, Johnson M, Jeeva S, Renisheya Joy Jeba Malar T

The present study was aimed to scrutinize the phytochemical components present in the various extracts of Asystasia gangetica (L.) T. Anderson using FT-IR, TLC and GC-MS. Preliminary phytochemical screening of the extracts was carried out using the standard method. The crude powder of A. gangetica was passed into the FT-IR and the peak values were recorded. TLC profile for phenolics and steroids was done using the mobile phase chloroform and methanol (9:1) and benzene and methanol at 9:1 ratio respectively. GC-MS analysis was performed on the benzene extracts to find out the chemical constituents. Preliminary phytochemical investigation confirmed the presence of various primary and secondary metabolites with varied degree. FT-IR spectrum revealed the functional constituents present in the crude powder of A. gangetica. TLC profile showed distinct bands with varied Rf values. GC-MS analysis results leads to the identification of 27 different compounds. The findings of the present study recommended the use of A. gangetica for developing plant based drugs for various ailments. Further study is recommended on other constituents on a road map of development of other phyto-pharmaceuticals for disease management.