Journal of Harmonized Research in Pharmacy

PHYTOCHEMICAL, CYTOTOXIC INVESTIGATION AND MOLECULAR DOCKING STUDIES OF CYTISUS SCOPARIUS Link FOR ITS DUAL HUMAN TOPO POISONING (I & II) ACTIVITY.

Abstract

Author(s): M. V. N. L. Chaitanya*, S. P. Dhanabal, S. Jubie & M.Pavithra

• The aerial parts of weed plant Cytisus scoparius (L.)Links have good amounts of phenolics, flavanoids and saponins. • A novel phenolic compound (1E)-1-hydroxyprop-1-en-2-yl (2E)-3-(4-h ydroxy-3-methoxyphenyl)prop-2-enoate (CH2) was isolated and characterized first time from this plant using Flash chromatography. • First time, the total saponin fraction was isolated and characterized by LCMS finger printing and found to be have ruscogenin as one of the saponin • The total saponin fractions and isolated compound showed significant antioxidant and anticancer properties. • To study their mechanism of action for cytotoxicity these molecules have been docked into the crystal structure of Topo –I and Topo-II enzymes usingSchrödinger suite, 2014-3. COMPOUND SELECTED FOR THE PRESENT STUDY S.NO Name of the compounds (molecular weight) Structure 1. (1E)-1-hydroxyprop-1-en-2-yl (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate (CH2) ( 250.2) 2. Ruscogenin (430.6) •